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. 2005 Aug 10;33(14):4544–4552. doi: 10.1093/nar/gki766

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Experimental strategy. For the creation of double mutant cell lines, we used rad51d cells expressing a mouse RAD51D cDNA together with the Cre-loxP system (30,31) (rad51d/RAD51D cells). Two rad51d/RAD51D clones were transfected with gene disruption constructs to obtain rad51b/rad51d/RAD51D and xrcc3/rad51d/RAD51D clones. After 3 days treatment of OH-TAM, we isolated rad51b/rad51d and xrcc3/rad51d mutant clones.