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. 2005 Aug 10;33(14):4544–4552. doi: 10.1093/nar/gki766

Figure 3.

Figure 3

(A) Percent colony survival assay after treatment with γ-irradiation. Comparison of wild-type cells with rad51b, rad51d, xrcc3, rad51b/rad51d and xrcc3/rad51d cells. The data shown are mean ± SD of at least three separate experiments. Two independently targeted clones of each genotype consistently showed the same sensitivity to genotoxic agents (data not shown). The kinetics of Rad51 and Rad54 foci formation after genotoxic treatments (B–E). The number of Rad51 foci per cell is displayed at the time point of 0, 2, 4, 8 and 12 h after (B) IR (4Gy) and 0, 2, 5, 8 and 11 h after (D) CDDP (15 μM, 1 h). Similarly, the number of Rad54 foci per cell is shown after exposure to (C) IR and (E) CDDP. Each result represents data of scoring at least 50 cells. Symbol of each mutant: WT (gray filled diamond, solid line), rad51b (blue filled circle, dotted line), rad51d (blue filled triangle, dotted line), xrcc3 (blue filled square, dotted line), rad51b/rad51d (green open circle, solid line) and xrcc3/rad51d (red open triangle, solid line).