Figure 4. Expression patterns of (a) ff1 genes during early development, and (b) ff1c and (c) ff1d in different adult tissues detected by RT-PCR.

RNA was isolated from different stages or from adult tissues, and primers for specific ff1 genes were used for RT-PCR. The abbreviations for the developmental stages are as follows: C, cleavage; B, blastula, G, gastrula, S, segmentation, P, pharyngula. 1.5, 2, 4 and 6 represent the number of days post-fertilization. N, negative control. Expressions of ff1a and ff1b genes begin during segmentation and become more evident during pharyngula. The ff1c gene is expressed starting at 4 days post-fertilization. Transcripts of ff1d start maternally at the cleavage stage and persist throughout all examined time points. Actin expression was used as a control.