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. 2005 Jun 21;389(Pt 1):57–62. doi: 10.1042/BJ20050213

Figure 1. Immunodetection of G-6-PT protein in rat liver microsomes.

Figure 1

Rat liver microsomal proteins (40 μg) were separated by SDS/11% PAGE and blotted on to a nitrocellulose membrane. The blots were reacted either with the whole immunoserum against the three peptides P1, P2 and P3 (lane 2) or the purified antibodies against P1 (lane 3), P2 (lane 4) or P3 (lane 5) respectively. The blot on lane 1 was reacted with the pre-immunoserum. The immunoserum and the purified antibodies were produced as detailed in the Experimental section. The sizes of molecular mass markers (in kDa) are shown.