Figure 4. HPLC analysis of BxLAM16A action on laminarin (0.25%, w/v).
Traces A, B, C, D and E, the enzyme (0.65 μg/ml) was incubated at 40 °C for 0, 3, 10, 210 min and overnight respectively. Samples were analysed on SUGAR KS-802 column ×2 (Shodex). Identified products are glucose (1), laminaribiose (2), laminari-oligomers (3–6) and laminarin (L).