Table 1. Substrate specificity of purified recombinant BxLAM16A.

The enzyme reaction was performed at 40 °C for 10 min by incubating 0.065 μg of purified enzyme with 0.25% (w/v) substrate in a final 0.1 ml of buffer solution. The results are means for three independent experiments±S.D. No activity was detected in the case of pustulan, carboxymethylcellulose, glycol chitin, xylan, agarose, κ-carrageenan and xyloglucan. For xyloglucan, xyloglucan endotransferase activity was measured as described in the Experimental section.

Substrate	Specific activity (units/mg)
Laminarin	337±21
Yeast glucan	57±7
Barley β-D-glucan	10±4
Lichenan	7±3