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. 2005 Jun 21;389(Pt 1):151–159. doi: 10.1042/BJ20050068

Figure 2. Determination of SERCA isoforms using ratio PCR.

Figure 2

Mouse (A), rat (B), rabbit (C) and pig (D) were analysed in parallel. The amount of cDNA used for RT was adjusted to yield equal amounts of total SERCA. Following PCR, restriction digestion (see enzymes in Supplementary Table A at http://www.BiochemJ.org/bj/389/bj3890151add.htm) was performed to discriminate between SERCA1 and SERCA2. The panels in (I) show representative examples of ratio PCRs on cDNA of different muscle types (V, ventricles; A, atria; S, soleus; E, EDL). The panels in (II) summarize the data expressed as the means (±S.E.M.) of percentage SERCA1/total SERCA (grey) or percentage SERCA2/total SERCA (white) mRNA ratios. Mouse tissue was pooled from six different animals to prepare total RNA (n=2). For other species, n=3. VENTR, ventricles.