Table 1. Sequence of the primers used for site-directed mutagenesis.

Bases corresponding to mutated codons are underlined.

Mutant	Primer sequence
A209H top	5′-ATGGTGCCTATGCGCATGTCATACCAAAATG-3′
A209H bottom	5′-CATTTTGGTATGACATGCGCATAGGCACCAT-3′
A209N top	5′-ATGGTGCCTATGCGAATGTCATACCAAAATG-3′
A209N bottom	5′-CATTTTGGTATGACATTCGCATAGGCACCAT-3′
G102K top	5′-CATCAAGCTGCCTTGAAGTCGGTACCGCGTTC-3′
G102K bottom	5′-GAACGCGGTACCGACTTCAAGGCAGCTTGATG-3′
S306Y top	5′-GGATGTCGTCACTACCTCGCTGATATCAG-3′
S306Y bottom	5′-CTGATATCAGCGAGGTAGTGACGTACATCC-3′
Q201E top	5′-GTGTTCGGTCGTCGAGAAGATCCCAATGGTGC-3′
Q201E bottom	5′-GTTACACAAGCCAGCAGCTCTTCTAGGGTTACCACG-3′
S143A top	5′-CACTTATGCGGCAAGTGCCTCTACCTATGGAGATC-3′
S143A bottom	5′-GATCTCCATAGGTAGAGGCACTTGCCGCATAAGTG-3′
S144K top	5′-CTTATGCGGCAAGTAGCAAGACCTATGGAGATCATC-3′
S144K bottom	5′-GATGATCTCCATAGGTCTTGCTACTTGCCGCATAAG-3′