Table 2. Summary of the effects of the mutations analysed in the present study on the substrate specificity of WbpP.
| Catalysis on | ||||||
|---|---|---|---|---|---|---|
| Mutant | UDP-GlcNAc | UDP-GalNAc | UDP-Glc | UDP-Gal | Specificity expected | Specificity observed |
| A209H | Very limited | Very limited | Slightly reduced | Slightly reduced | Group 1a | Group 2 |
| A209N | Slightly reduced | Slightly reduced | Very limited | Very limited | Group 2 | Enhanced group 3 |
| G102K | Slightly reduced | Slightly reduced | Abolished | Abolished | None | Enhanced group 3 |
| Q201E | Maintained | Maintained | Very limited | Very limited | None | Enhanced group 3 |
| Q201E/G102K | Slightly reduced | Slightly reduced | Abolished | Very limited | Group 2 | Enhanced group 3 |
| S143A | Maintained | Maintained | Abolished | Abolished | Enhanced group 3 | Enhanced group 3 |
| S144K | Abolished | Abolished | Abolished | Abolished | Group 2 or dehydration | Not applicable |