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The fluorescence of membrane-bound Rh-CT3Nk (a) was bleached with a laser in the restricted region indicated by the arrow (b). No fluorescence recovery due to Rh-CT3Nk lateral diffusion was observed 15 min (c) or 25 min (d) after photobleaching. Cells were incubated with 0.2 μM Rh-CT3Nk for 1 h.
