. 2024 Oct 30;46(3):653–661. doi: 10.1038/s41401-024-01408-3

Table 1.

Primers used for RT-PCR.

Primer name	Sequence (5′-3′)
GAPDH	Forward	CATGTTCGTCATGGGGTGAACCA
GAPDH	Reverse	AGTGATGGCATGGACTGTGGTCAT
HNF1α	Forward	CCTGTCCCAACACCTCAACAA
HNF1α	Reverse	TTGAAACGGTTCCTCCGC
HBV pgRNA	Forward	CTCAATCTCGGGAATCTCAATGT
HBV pgRNA	Reverse	TGGATAAAACCTAGCAGGCATAAT
^aTotal HBV-specific transcripts	Forward	ATCCTGCTGCTATGCCTCATCTT
^aTotal HBV-specific transcripts	Reverse	ACAGTGGGGGAAAGCCCTACGAA
HBV-DNA	Forward	TCACCAGCACCATGCAAC
HBV-DNA	Reverse	AAGCCACCCAAGGCACAG
hB1F	Forward	GGCTTATGTGCAAAATGGCAGATC
hB1F	Reverse	GCTCACTCCAGCAGTTCTGAAG
pS2	Forward	CCAGGCCCAGGAAGAAACAT
pS2	Reverse	AACAGCAACCTCTCTCCGTG
C/EBPα	Forward	TGGACAAGAACAGCAACGAGTA
C/EBPα	Reverse	ATTGTCACTGGTCAGCTCCAG
Hepcidin	Forward	CTCCTTCGCCTCTGGAACAT
Hepcidin	Reverse	AGTGGCTCTGTTTTCCCACA
CyclinD1	Forward	TGTTCGTGGCCTCTAAGATGAAG
CyclinD1	Reverse	AGGTTCCACTTGAGCTTGTTCAC

^aTotal HBV-specific transcripts: Designed based on all RNA molecules transcribed in HBV.