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(A) Agarose gel electrophoresis of HBsu and HBsu-D26A binding to supercoiled (s/c; top panel; nc refers to nicked circle) or linear (lin; bottom panel) pUC18 DNA. (B) HBsu and HBsu-D26A binding to nicked pET5a DNA. HBsu variants are identified at the bottom, and protein concentrations, identical for both panels, are indicated below the bottom panel.
