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(A) HBsu and (B) HBsu-D26 binding to 1 nM four-way junction DNA. Protein concentrations, identical for both panels, are 0.06, 0.12, 0.25, 0.5, 0.75, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 8.0 and 10 nM. Reactions were analysed on 6% native gels. The essentially quantitative conversion of available DNA to complex at 1 nM protein (lanes 7) indicates that reactions are performed under stoichiometric conditions (Kd<[DNA]).
