Poly(A)+ RNA was extracted from the kidneys of VDR(+/+) and VDR(−/−) mice that had been injected with FGF23WT, FGF23M or the empty vector. Each lane was loaded with 3 μg of RNA. Upper panels, type IIa co-transporter; middle panels, type IIc Na/Pi co-transporter; lower panels, GAPDH (internal control). The visualized band intensity for the mice injected with the empty vector expression was designated as 1.0, and all other band intensities were expressed relative to this value. One-way ANOVA (post hoc Scheffé F-test) and two-factor factorial ANOVA were performed. Values are means±S.E.M. (n=5–6).