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. 2005 Aug 9;390(Pt 1):325–331. doi: 10.1042/BJ20041799

Figure 4. Effects of injection of FGF23M on 1α(OH)ase and 24(OH)ase mRNA levels.

Figure 4

Poly(A)+ RNA was extracted from the kidneys of VDR(+/+) and VDR(−/−) mice that had been injected with FGF23WT, FGF23M or the empty vector. Each lane was loaded with 3 μg of RNA. Upper panel, 1α(OH)ase; middle panel, 24(OH)ase; lower panel, GAPDH (internal control). In the histograms, the visualized band intensity for the VDR(+/+) mice injected with the empty vector was designated as 1.0, and all other band intensities are expressed relative to this value. One-way ANOVA (post hoc Scheffé F-test) and two-factor factorial ANOVA were performed. Values are means±S.E.M. (n=5–6); ap<0.05 compared with VDR(+/+) mock vector control., bp<0.05 compared with VDR(−/−) mock vector control.