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. 2005 Aug 16;3(9):e283. doi: 10.1371/journal.pbio.0030283

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Copyright: © 2005 Conti et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Derivation from human ES cells: human ES cell primary culture (a), differentiation of human ES cells into neural-rosette structures (b), passage 9 in NS expansion medium (c), individual cells exhibit radial glial morphology (d), and immunostaining for NS cell/radial glia markers (e–h).

(B) Derivation from human foetal forebrain: floating clusters (i) generated from cortex, attachment and outgrowth (j), passage 5 in NS expansion medium (k), radial glia morphology (l), and NS cell/radial glial markers (m–p).

(C) Differentiation of human foetal NS cells: TuJ1 positive neuronal cells generated by sequential growth factor withdrawal (q), and GFAP positive astrocytes induced by exposure to serum (r).