Abstract
1. A procedure for preparing crystalline 3-phosphoglycerate kinase from rabbit or pig skeletal muscle is presented. 2. The preparation phosphorylates up to 975μmoles of 3-phosphoglycerate/min./mg. at 30° and is not contaminated with myokinase. 3. The enzyme has an estimated molecular weight of 36500±1000, and contains three residues each of tyrosine and tryptophan. 4. The preparation is suitable for use in the enzymic procedures for determining ATP, phosphocreatine and 3-phosphoglycerate.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Avramov I. A., Repin V. S. Vydelenie vysokoochishchennoi fosfoglitseratkinazy iz skeletnykh mushts krolika. Biokhimiia. 1965 Nov-Dec;30(6):1187–1193. [PubMed] [Google Scholar]
- BEAVEN G. H., HOLIDAY E. R. Ultraviolet absorption spectra of proteins and amino acids. Adv Protein Chem. 1952;7:319–386. doi: 10.1016/s0065-3233(08)60022-4. [DOI] [PubMed] [Google Scholar]
- FISCHER E. H., KREBS E. G. The isolation and crystallization of rabbit skeletal muscle phosphorylase b. J Biol Chem. 1958 Mar;231(1):65–71. [PubMed] [Google Scholar]
- GOLDFARB A. R., SAIDEL L. J., MOSOVICH E. The ultraviolet absorption spectra of proteins. J Biol Chem. 1951 Nov;193(1):397–404. [PubMed] [Google Scholar]
- GOSSELIN-REY C. Purification and properties of phosphoglycerate kinase from chicken breast muscle. Biochim Biophys Acta. 1963 Jan 8;67:140–142. [PubMed] [Google Scholar]
- Goodwin T. W., Morton R. A. The spectrophotometric determination of tyrosine and tryptophan in proteins. Biochem J. 1946;40(5-6):628–632. doi: 10.1042/bj0400628. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HASHIMOTO T., YOSHIKAWA H. Crystalline phosphoglycerate kinase from human erythrocytes. Biochim Biophys Acta. 1962 Dec 4;65:355–357. doi: 10.1016/0006-3002(62)91057-0. [DOI] [PubMed] [Google Scholar]
- JECSAI G. Crystalline lactic dehydrogenase from pig skeletal muscle. Acta Physiol Acad Sci Hung. 1961;20:339–346. [PubMed] [Google Scholar]
- Parmeggiani A., Luft J. H., Love D. S., Krebs E. G. Crystallization and properties of rabbit skeletal muscle phosphofructokinase. J Biol Chem. 1966 Oct 25;241(20):4625–4637. [PubMed] [Google Scholar]
- Scopes R. K. Isolation and properties of a basic protein from skeletal-muscle sarcoplasm. Biochem J. 1966 Jan;98(1):193–197. doi: 10.1042/bj0980193. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scopes R. K. Methods for starch-gel electrophoresis of sarcoplasmic proteins. An investigation of the relative mobilities of the glycolytic enzymes from the muscles of a variety of species. Biochem J. 1968 Mar;107(2):139–150. doi: 10.1042/bj1070139. [DOI] [PMC free article] [PubMed] [Google Scholar]