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. 2025 Feb 22;8:278. doi: 10.1038/s42003-025-07662-3

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 7 — a Coimmunoprecipitation of cyclin A or HA-Cdh1 with Flag-Skp2 in 293 T cells overexpressing Flag-Skp2, with (lanes 4–6) or without (lanes 1–3) Myc-p27 overexpression. b–g 293 T cells were transfected with Flag-Skp2 (lanes 1–6), HA-Cdh1 (lanes 3–6), or Myc-p27 (lanes 5, 6), as indicated. All groups were treated with and without MG132. After IP of Skp2 with an anti-mouse Flag antibody, its ubiquitinated forms were detected with an anti-rabbit ubiquitin antibody (c). Ubiquitinated Flag-tagged Skp2 has an estimated molecular weight of 58-63 kDa. Total cell extracts were probed with an anti-mouse Flag (d), anti-rabbit HA (e), anti-mouse Myc (f), or anti-mouse tubulin antibodies (g).