Table 3.

Serological analysis of bat samples. All samples were screened by BMBA using the recombinant proteins expressed in L. tarentolae (lt). Reactive samples were repeated in the BMBA using the in-house (lt) as well as commercially available (c) proteins, and in the indirect ELISA using the in-house (lt) proteins as well as commercially available (c) proteins

NiV G Lt Nipah virus glycoprotein expressed in Leishmania tarentolae, HeV G Lt Hendra virus glycoprotein expressed in L. tarentolae, GhV G Lt Ghana virus glycoprotein expressed in L. tarentolae, NiV G-c Commercially available NiV G, HeV G-c Commercially available HeV G, Values under BMBA represent the Median Fluorescence Intensity (MFI) and values under ELISA represent Optical Density (OD)

-1- first analysis, -2- repeated analysis,neg con Negative control, pos con Positive control, It needs to be kept in mind that the control samples were sera that were collected after an optimized prime / boost protocol under experimental conditions and immediately stored frozen, while the field samples analyzed here are transudates collected from dead animals of which the timepoint of exposure to the antigen is completely unclear

Red:result > cutoff value