Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Leishmania mexicana) | L. mexicana wildtype | Eva Gluenz laboratory | WHO strain MNYC/BZ/62/M379 | Used TriTrypDB (release 59, Aslett et al., 2010) reference annotation: L. mexicana MHOMGT2001U1103 |
| Cell line (Leishmania major) |
L. major wildtype | Eva Gluenz laboratory | Strain Friedlin | Used TriTrypDB (release 59, Aslett et al., 2010) reference annotation: L. major Friedlin |
| Cell line (Leishmania donovani) |
L. donovani wildtype | Joachim Clos laboratory (Decuypere et al., 2005) | Strain BPK190 | Used TriTrypDB (release 59, Aslett et al., 2010) reference annotation: L. donovani BPK282A1 |
| Recombinant DNA reagent | pTB007-hyBE4max | Engstler and Beneke, 2023 | Contains CBE hyBE4max and T7 RNAP | |
| Recombinant DNA reagent | pLdCH-hyBE4max | Engstler and Beneke, 2023 | Contains CBE hyBE4max and CBE sgRNA | |
| Recombinant DNA reagent | pLdCH-hyBE4max-LmajDBD | Engstler and Beneke, 2023 | Contains Leishmania-optimized CBE hyBE4max and CBE sgRNA | |
| Recombinant DNA reagent | pTB107 | This study | Contains CBE hyBE4max, AsCas12a ultra, and T7 RNAP (available in versions with either a hygromycin or phleomycin-resistance marker) |
|
| Recombinant DNA reagent | pTB106 | This study | Contains Leishmania-optimized CBE hyBE4max, AsCas12a ultra, and T7 RNAP (available in versions with either a hygromycin or phleomycin-resistance marker) |
|
| Recombinant DNA reagent | pTB102 | This study | Contains T7 RNAP promoter-driven CBE sgRNA expression cassette without homology flanks | |
| Recombinant DNA reagent | pTB104 | This study | Contains T7 RNAP promoter-driven CBE sgRNA expression cassette with homology flanks for neomycin-resistance gene | |
| Recombinant DNA reagent | pTB105 | This study | Contains T7 RNAP promoter-driven CBE sgRNA expression cassette with homology flanks for 18S rRNA SSU locus | |
| Software | TriTrypDB (release 59) | Aslett et al., 2010 | https://tritrypdb.org/tritrypdb/app | |
| Software | LeishBASEedit | This study | http://www.leishbaseedit.net/ | See description under ‘Automated CBE guide design using LeishBASEedit’ |
| Gene (L. donovani BPK282A1, L. major Friedlin, L. mexicana MHOMGT2001U1103) | PF16 | TriTrypDB (release 59) (Aslett et al., 2010) | LdBPK_201450.1, LmjF.20.1400, LmxM.20.1400 |