Abstract
A method is described for isolation of the acid-hydrolysable metabolite of aldosterone in sufficient purity to measure accurately the daily production rate. Values obtained with six hospital patients were 84–131μg./day on a daily intake of 100m-equiv. of Na+ and 227–464μg./day on a daily intake of 10m-equiv. of Na+. Corresponding values for aldosterone excretion were also recorded, but these are a poor index of production rate since they represent from 1·6 to 9·8% of the total daily output of aldosterone.
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- BURTON R. B., ZAFFARONI, KEUTMANN E. H. Paper chromatography of steroids. II. Corticosteroids and related compounds. J Biol Chem. 1951 Feb;188(2):763–771. [PubMed] [Google Scholar]
- BUSH I. E. Methods of paper chromatography of steroids applicable to the study of steroids in mammalian blood and tissues. Biochem J. 1952 Jan;50(3):370–378. doi: 10.1042/bj0500370. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COPE C. L., NICOLIS G., FRASER B. Measurement of aldosterone secretion rate in man by the use of a metabolite. Clin Sci. 1961 Dec;21:367–380. [PubMed] [Google Scholar]
- Espiner E. A., Tucci J. R., Jagger P. I., Pauk G. L., Lauler D. P. The effect of acute diuretic-induced extracellular volume depletion on aldosterone secretion in normal man. Clin Sci. 1967 Aug;33(1):125–134. [PubMed] [Google Scholar]
- FLOOD C., LAYNE D. S., RAMCHARAN S., ROSSIPAL E., TAIT J. F., TAIT S. A. An investigation of the urinary metabolites and secretion rates of aldosterone and cortisol in man and a description of methods for their measurement. Acta Endocrinol (Copenh) 1961 Feb;36:237–264. doi: 10.1530/acta.0.0360237. [DOI] [PubMed] [Google Scholar]
- GENEST J., JONES R. N., KOIW E., NOWACZYNSKI W. J., STEYERMARK P. R. Detailed study of a purified urinary aldosterone fraction. Can J Biochem Physiol. 1956 Sep;34(5):1023–1038. [PubMed] [Google Scholar]
- GENEST J., KOIW E., NOWACZYNSKI W., SANDOR T. Study of a large steroid spectrum in normal subjects and hypertensive patients. Acta Endocrinol (Copenh) 1960 Nov;35:413–425. doi: 10.1530/acta.0.xxxv0413. [DOI] [PubMed] [Google Scholar]
- JONES K. M., LLOYD-JONES R., RIONDEL A., TAIT J. F., TAIT S. A., BULBROOK R. D., GREENWOOD F. C. Aldosterone secretion and metabolism in normal men and women and in pregnancy. Acta Endocrinol (Copenh) 1959 Mar;30(3):321–342. doi: 10.1530/acta.0.0300321. [DOI] [PubMed] [Google Scholar]
- KLIMAN B., PETERSON R. E. Double isotope derivative assay of aldosterone in biological extracts. J Biol Chem. 1960 Jun;235:1639–1648. [PubMed] [Google Scholar]
- NEHER R., WETTSTEIN A. Physicochemical estimation of aldosterone in urine. J Clin Invest. 1956 Jul;35(7):800–805. doi: 10.1172/JCI103332. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SIEGENTHALER W. E., DOWDY A., LUETSCHER J. A. Determination of the secretion rate of aldosterone in normal man by use of 7-H3-d-aldosterone and acid hydrolysis of urine. J Clin Endocrinol Metab. 1962 Feb;22:172–177. doi: 10.1210/jcem-22-2-172. [DOI] [PubMed] [Google Scholar]
- TAIT J. F., TAIT S. A., LITTLE B., LAUMAS K. R. The disappearance of 7-H-3-d-aldosterone in the plasma of normal subjects. J Clin Invest. 1961 Jan;40:72–80. doi: 10.1172/JCI104239. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ULICK S., LARAGH J. H., LIEBERMAN S. The isolation of a urinary metabolite of aldosterone and its use to measure the rate of secretion of aldosterone by the adrenal cortex of man. Trans Assoc Am Physicians. 1958;71:225–235. [PubMed] [Google Scholar]