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. 2025 Feb 6;14(2):164. doi: 10.3390/biology14020164

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© 2025 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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BGCs detected in TRM70308^T. (A) The Venn diagram shows the overlap of experimentally validated biosynthetic gene clusters (BGCs) in the MIBiG database with those from TRM70308^T. Only BGC009 from TRM70308^T overlaps with a reference BGC (Ficellomycin), forming a gene cluster family (GCF), s56-p1 represent the gene cluster corresponding to BGC009 in antiSMASH. (B) An evolutionary analysis of BGCs that overlap with TRM70308^T BGC009, based on data from the MIBiG database and antiSMASH prediction results, bootstrap values (expressed as percentages of 1000 replications) of above 50% are shown at branch points.