Figure 1.

This graphical abstract shows the experimental design and methods used in this study. The mice transplanted with human adult fecal microbiota were fed fluorescently labeled donor E. coli bacteria carrying the GFP-encoded RP4 plasmid. The colonization of donor bacteria was assessed by culturing bacteria directly from mouse fecal samples. FACS was performed on fecal samples to detect and sort transconjugant bacteria expressing only GFP encoded on the RP4 plasmid. 16S rRNA amplicon sequencing was carried out on FACS bacteria to characterize the potential recipients of the broad host range RP4 plasmid in the gut microbiota. Created with BioRender.com.