Figure 5.

Validation of RNA sequencing by real-time PCR of DETs. The following DETs were selected to validate RNA-seq by real-time PCR: tyr, crebl, ddc, maob, abcb1, mfng, rab11a, cacna1, calm1. For RNA-seq, in blue, “#” indicates a log2 fold change ≥2.0 and FDR <0.05. For RT-qPCR, in orange, relative expression was normalized against fau and actβ. Fold change from control indicates the relation between dark edge transcript expression and white edge transcript expression. The results are expressed as means and + standard errors (n = 3 per group). Differences between DME and WME groups are shown in * p < 0.05, and ** p < 0.01.