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. 1969 Dec;115(5):969–976. doi: 10.1042/bj1150969

The metabolism of d-glucarate by Pseudomonas acidovorans

R Jeffcoat 1, H Hassall 1, S Dagley 1
PMCID: PMC1185239  PMID: 4311826

Abstract

1. Dehydratases that converted d-glucarate into 4-deoxy-5-oxoglucarate were partially purified from Klebsiella aerogenes and Pseudomonas acidovorans. 2. When d-glucarate was metabolized to 2,5-dioxovalerate it appeared that water and carbon dioxide were removed from 4-deoxy-5-oxoglucarate in one enzymic step: 4,5-dihydroxy-2-oxovalerate was not an intermediate in this reaction. 3. A method for the enzymic determination of d-glucarate is described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BACHRACH U. The aerobic breakdown of uric acid by certain pseudomonads. J Gen Microbiol. 1957 Aug;17(1):1–11. doi: 10.1099/00221287-17-1-1. [DOI] [PubMed] [Google Scholar]
  2. BLUMENTHAL H. J., FISH D. C. Bacterial conversion of D-glucarate to glycerate and pyruvate. Biochem Biophys Res Commun. 1963 May 3;11:239–243. doi: 10.1016/0006-291x(63)90341-3. [DOI] [PubMed] [Google Scholar]
  3. Bayly R. C., Dagley S., Gibson D. T. The metabolism of cresols by species of Pseudomonas. Biochem J. 1966 Nov;101(2):293–301. doi: 10.1042/bj1010293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blumenthal H. J., Jepson T. Asymmetric dehydration of galactarate by bacterial galactarate dehydratase. Biochem Biophys Res Commun. 1964 Oct 14;17(3):282–287. doi: 10.1016/0006-291x(64)90398-5. [DOI] [PubMed] [Google Scholar]
  5. Chamberlain E. M., Dagley S. The metabolism of thymol by a Pseudomonas. Biochem J. 1968 Dec;110(4):755–763. doi: 10.1042/bj1100755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DAGLEY S., TRUDGILL P. W., CALLELY A. G. Synthesis of cell constituents from glycine by a Pseudomonas. Biochem J. 1961 Dec;81:623–631. doi: 10.1042/bj0810623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DAGLEY S., TRUDGILL P. W. THE METABOLISM OF GALACTARATE, D-GLUCARATE AND VARIOUS PENTOSES BY SPECIES OF PSEUDOMONAS. Biochem J. 1965 Apr;95:48–58. doi: 10.1042/bj0950048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DAGLEY S., TRUDGILL P. W. THE METABOLISM OF TARTARIC ACID BY A PSEUDOMONAS. A NEW PATHWAY. Biochem J. 1963 Oct;89:22–31. doi: 10.1042/bj0890022. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dagley S., Geary P. J., Wood J. M. The metabolism of protocatechuate by Pseudomonas testosteroni. Biochem J. 1968 Oct;109(4):559–568. doi: 10.1042/bj1090559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FEINBERG R. H., GREENBERG D. M. Studies on the enzymic decomposition of urocanic acid. J Biol Chem. 1959 Oct;234:2670–2676. [PubMed] [Google Scholar]
  11. HUGHES D. E. A press for disrupting bacteria and other micro-organisms. Br J Exp Pathol. 1951 Apr;32(2):97–109. [PMC free article] [PubMed] [Google Scholar]
  12. Ishidate M., Matsui M., Okada M. Biochemical studies on glucuronic acid and glucaric acid. 1. Quantitative chemical determination of D-glucaric acid in urine. Anal Biochem. 1965 May;11(2):176–189. doi: 10.1016/0003-2697(65)90004-7. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Marsh C. A. Metabolism of d-glucuronolactone in mammalian systems. Identification of d-glucaric acid as a normal constituent of urine. Biochem J. 1963 Jan;86(1):77–86. doi: 10.1042/bj0860077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Portsmouth D., Stoolmiller A. C., Abeles R. H. Studies on the mechanism of action of 2-keto-3-deoxy-L-arabonate dehydratase. The participation of an enzyme-substrate Schiff base in a dehydration. J Biol Chem. 1967 Jun 10;242(11):2751–2759. [PubMed] [Google Scholar]
  16. STOOLMILLER A. C., ABELES R. H. FORMATION OF ALPHA-KETOGLUTARATE SEMIALDEHYDE. AN INTRAMOLECULAR DISMUTATION REACTION. Biochem Biophys Res Commun. 1965 May 3;19:438–443. doi: 10.1016/0006-291x(65)90143-9. [DOI] [PubMed] [Google Scholar]
  17. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
  18. Stoolmiller A. C., Abeles R. H. Formation of alpha-ketoglutaric semialdehyde from L-2-keto-3-deoxyarabonic acid and isolation of L-2-keto-3-deoxyarabonate dehydratase from Pseudomonas saccharophila. J Biol Chem. 1966 Dec 25;241(24):5764–5771. [PubMed] [Google Scholar]
  19. Trudgill P. W., Widdus R. D-glucarate catabolism by pseudomonadaceae and enterobacteriaceae. Nature. 1966 Sep 3;211(5053):1097–1099. doi: 10.1038/2111097a0. [DOI] [PubMed] [Google Scholar]
  20. WARREN L. Thiobarbituric acid spray reaction for deoxy sugars and sialic acids. Nature. 1960 Apr 16;186:237–237. doi: 10.1038/186237a0. [DOI] [PubMed] [Google Scholar]
  21. WEISSBACH A., HURWITZ J. The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification. J Biol Chem. 1959 Apr;234(4):705–709. [PubMed] [Google Scholar]

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