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. 2025 Feb 16;13(2):60. doi: 10.3390/diseases13020060

Figure 4.

Figure 4

Reactive oxygen species content and mitochondrial status in TREM2-overexpressing and wild-type cells after LPS treatment. The flow cytometry workflow is the top panel. Using different fluorescent dyes (DCFH-DA, MitoSOX, and MitoBright) as probes, flow cytometry was used to detect intracellular ROS (A), mitochondrial ROS (B), and mitochondrial content (C) in cells after LPS (1000 ng/mL) treatment for 4 h (n = 3). MFI stands for mean fluorescence intensity. (D) Representative transmission electron microscopy images of mitochondria in wild-type and (E) TREM2-overexpressing cells after LPS (1000 ng/mL) stimulation for 4 h. M represents mitochondria and N represents the nucleus. Scale bars: 2 μm and 500 nm (white). The images on the right are magnified views of the white boxes in the images on the left. The data are presented as means ± SDs. All results represent three independent experiments. ns: not significant, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.