Table 1.
Sequence of primers used in this study.
miRNAs/Genes | Sequence Information | Purpose | Fragment Length and Annealing Temperature | |
---|---|---|---|---|
Forward (5′–3′) | Reverse (5′–3″) | |||
miR-21 | CGCGTAGCTTATCAGACTGATGTTGAC | mRQ 3′ primer | RT-qPCR | 60 °C |
PDCD4 | GATGATGACCAGGAGAAC | TCCAACGCTAAGGACACT | 200 bp, 58 °C | |
18sRNA | GTGGTGTTGAGGAAAGCAGACA | TGATCACACGTTCCACCTCATC | 79 bp, 60 °C | |
U6 | ACGGACAGGATTGACAGATT | TCGCTCCACCAACTAAGAA | 80 bp, 58 °C | |
GAPDH | ATCTCGCTCCTGGAAGATG | TCGGAGTGAACGGATTCG | 173 bp, 58 °C | |
PDCD4 (WT) | CCGctcgagAGGGTGTAAAGGAGGGAC | ATTTgcggccgcGAAAGGAGTGGCAGTCAG | Construction of dual luciferase reporter vectors | 356 bp, 60 °C |
PDCD4 (Mut) | TTCTCtattcgaACCTTTTGTAAGTGCCATGTTTATG | AAGGTtcgaataGAGAATATCCCACTTAAGAAGTGGTTAC | 368 bp, 60 °C |