Figure 4.

Physiological characterization of an OmpJ-deficient mutant. The growth of the wild-type (WT) and OmpJ-deficient mutant (OmpJ-) strains with fumarate (A), Fe(III) citrate (B), and insoluble Fe(III) (C) or Mn(VI) (D) oxides was studied. Growth on fumarate was quantified by following the optical density of the culture at 600 nm (OD600), while growth with soluble or insoluble Fe(III) was monitored by measuring the levels of soluble Fe(II) present in the medium as a result of Fe(III) reduction. Error bars are the standard deviation from the average of triplicate determinations. The reduction of Mn(VI) oxides was visually tested as the oxidized Mn(VI) oxides turn from black to a whitish precipitate upon reduction. Uninoculated controls also were included in B–D.