Table 2.
The summary of commonly observed glycopeptides from α- and β -chain from four PMSG products1.
| Site | Observed m/z2 | [M + H]+ | Sequences3 | Glycan structures |
|---|---|---|---|---|
| α-chain | ||||
| N80 | 1192.1313+ | 3574.393 | K.NITSESTC*C*VAK | Core(HexNAcHex)2NeuAc2 |
| 1206.1413+ | 3616.423 | Core(HexNAcHex)2NeuAc2 + acetyl | ||
| 1220.1443+ | 3658.432 | Core(HexNAcHex)2NeuAc2 + 2acetyl | ||
| 1313.8443+ | 3939.532 | Core(HexNAcHex)3NeuAc2 | ||
| 1327.8503+ | 3981.550 | Core(HexNAcHex)3NeuAc2 + acetyl | ||
| 1341.8493+ | 4023.547 | Core(HexNAcHex)3NeuAc2 + 2acetyl | ||
| 1410.8713+ | 4230.613 | Core(HexNAcHex)3NeuAc3 | ||
| 1424.8723+ | 4272.616 | Core(HexNAcHex)3NeuAc3 + acetyl | ||
| 1438.8743+ | 4314.622 | Core(HexNAcHex)3NeuAc3 + 2acetyl | ||
| N106 | 1122.9304+ | 4488.720 | K.LENHTQCYC*STC*YHHK | CoreFuc(HexNAcHex)2NeuAc2 |
| 1133.4354+ | 4530.740 | CoreFuc(HexNAcHex)2NeuAc2 + acetyl | ||
| 915.3485+ | 4572.741 | CoreFuc(HexNAcHex)2NeuAc2 + 2acetyl | ||
| 1214.2114+ | 4853.844 | CoreFuc(HexNAcHex)3NeuAc2 | ||
| 1224.7184+ | 4895.872 | CoreFuc(HexNAcHex)3NeuAc2 + acetyl | ||
| 1286.9914+ | 5144.964 | CoreFuc(HexNAcHex)3NeuAc3 | ||
| β-chain | ||||
| N33 | 1126.8123+ | 3574.393 | R.PINATLAAEK.E | Core(HexNAcHex)2NeuAc2 |
| 1140.8153+ | 3420.4462 | Core(HexNAcHex)2NeuAc2 + acetyl | ||
| 1154.8183+ | 3462.4562 | Core(HexNAcHex)2NeuAc2 + 2acetyl | ||
1The results were the summary of data from both trypsin and GluC endopeptidase digestion. The identified glycopeptides in each sample were provided as supplementary Tables.
2The corresponding assignment was based on observed m/z from MS1 spectra although the quality of MS/MS spectra could not confirm the assignment.
3C* represents carbamidomethylated cysteines.