Figure 1.

4E-BP mutant flies are normal in size. (A) Newly hatched L1 larvae of genotypes w¹¹¹⁸ (“control”) and Thor² (“4E-BP null”) were seeded in vials at a density of 50/vial and grown at 25°C. Male adult flies were then aged 3 d and weighed in batches of 40. Average fly weights for control and 4E-BP-null flies were 0.75 and 0.74 mg, respectively. Error bars indicate standard deviations of 0.005 and 0.002 mg/fly, respectively. (B) Quantitative real-time RT–PCR was performed on RNA extracted from indicated tissues of wandering third instar larvae. The values shown are 4E-BP transcript levels normalized to rp49 transcript levels. Error bars show standard deviation on the assay done in triplicate.