Figure 2.

Stress induction of 4E-BP expression is FOXO-dependent. Quantitative real-time RT–PCR on RNA from whole adult control w¹¹¹⁸ flies (A) or FOXO^21/25 mutant flies (B). Larvae were grown under controlled conditions. Adult males were aged for 3 d and then placed in vials containing 0.8% agarose in PBS (“starved”) or supplemented with 10% sucrose or 10%sucrose + 5% H₂O₂. The values shown are 4E-BP transcript levels normalized to rp49 transcript levels. Error bars show standard deviation on the assay done in triplicate.