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. 2005 Aug 15;19(16):1894–1904. doi: 10.1101/gad.1336305

Figure 5.

Figure 5.

Genetic and functional interactions between Δisy1 and U6 alleles connected to prp16-302 activity. (A) Serial dilutions of the indicated strains were spotted on YPD plates and grown for 3 d at 25°C or at 37°C. (B) Primer extension analysis of RP51A RNA from indicated strains following growth at 25°C or a shift for 2.5 h to 37°C. RNA species are indicated schematically on the left. U14 snoRNA was used as a loading standard. The bottom portions of the gel are shown at a lighter exposure than the top part. Strains are denoted above lanes as follows: (Δ) Δisy1; (+) ISY1; (wt) U6; (43) U6-ΔC43; (44) U6-ΔA44; (A) U6-U57A; (C) U6-U57C. (C) Primer extension analysis of U3 snoRNA from indicated strains following growth at 25°C. PGK1 was used as a loading standard. Strains are denoted above the lanes as in B.