Table 3.

Activating ras mutations in epidermal tumors

		H-ras		K-ras
Treatment/mouse	Tumor type	Codons 12-13	Codons 59-61	Codons 12-13	Codons 59-61
DMBA + PMA
Wildtype-1	Pap	wt	GGTCTAGAA	wt	wt
Wildtype-2	Pap	wt	GGTCTAGAA	wt	wt
Wildtype-3	SCC	wt	GGTCTAGAA	wt	wt
Wildtype-4	Pap	wt	wt	wt	GGTCTAGAG
Wildtype-5	Pap	wt	GGTCTAGAA	N/A	wt
Wildtype-6	Pap	wt	GGTCTAGAA	N/A	wt
K5—Mat-B-1	Pap	wt	GGTCTAGAA	wt	wt
K5—Mat-B-2	Pap	wt	GGTCTAGAA	wt	wt
K5—Mat-B-3	Pap	wt	GGTCTAGAA	wt	wt
K5—Mat-B-4	SCC	wt	wt	wt	GGTCTAGAG
K5—Mat-B-5	SCC	wt	wt	wt	wt
K5—Mat-B-6	Pap	wt	GGTCTAGAA	wt	wt
DMBA
K5—Mat-B-7	SCC	wt	GGTCTAGAA	N/A	wt
K5—Mat-B-8	SCC	wt	GGTCTAGAA	N/A	N/A
K5—Mat-B-9	SCC	wt	GGTCTAGAA	N/A	N/A
K5—Mat-B-10	SCC	wt	wt	wt	wt
K5—Mat-B-11	SCC	wt	GGTCTAGAA	N/A	N/A
K5—Mat-B-12	SCC	wt	GGTCTAGAA	N/A	N/A
K5—Mat-B-13	SCC	wt	wt	wt	wt
K5—Mat-B-14	SCC	wt	wt	wt	wt
K5—Mat-B-15	SCC	wt	GGTCTAGAA	N/A	wt
K5—Mat-B-16	Pap	wt	GGTCTAGAA	wt	wt
Spontaneous tumors
K5—Mat-A-2	SCC	wt	wt	wt	wt
K5—Mat-A-4	SCC	wt	wt	wt	wt
K5—Mat-A-6	SCC	wt	wt	wt	wt
K5—Mat-A-7	SCC	wt	wt	wt	wt
K5—Mat-A-8	SCC/S	wt	wt	wt	wt
K5—Mat-A-9	SCC/S	wt	wt	wt	wt
K5—Mat-A-10	SCC	wt	wt	wt	wt

Tumors were analyzed by PCR amplification and sequencing (Ha-ras and K-ras codons 12-13) or PCR amplification followed by sequencing and XbaI restriction digest. (Pap) Papilloma; (SCC) squamous cell carcinoma; (SCC/S) mixed spindle-shaped carcinoma; (wt) wild-type allele.