Table 2. IC₁₀ values for U2OS cells treated with chemicals for 48 hours. The calculation was based on the logarithmic transformation of MTT assay results, followed by data normalization and curve fitting using the cubic spline method. The concentration corresponding to approximately 90% cell viability was identified and selected as the dose for subsequent PD experiments.

Compound	Cell survival (%)	IC10 concentration (μM L−1)
1	90.08	50
2	90.06	50
3	90.06	20
4	90.00	20
5	90.13	70
6	90.11	70
7	90.98	130
8	90.04	40
9	90.92	20
10	90.04	20