Table 2. Macromolecule-production information.
| Source organism | Homo sapiens |
| DNA source | Synthetic gene (IDT) |
| Expression vector | pET-11a |
| Plasmid construction method | In-Fusion cloning (Clontech) |
| Expression host | Escherichia coli BL21(DE3) |
| Expression details | Autoinduction medium (see the supporting information for details) |
| Complete amino-acid sequence of the protein produced (PXRLBD-Thb-SRC-1)† | MKKGHHHHHHGSERTGTQPLGVQGLTEEQRMMIRELMDAQMKTFDTTFSHFKNFRLPGVLSSGCELPESLQAPSREEAAKWSQVRKDLCSLKVSLQLRGEDGSVWNYKPPADSGGKEIFSLLPHMADMSTYMFKGIISFAKVISYFRDLPIEDQISLLKGAAFELCQLRFNTVFNAETGTWECGRLSYCLEDTAGGFQQLLLEPMLKFHYMLKKLQLHEEEYVLMQAISLFSPDRPGVLQHRVVDQLQEQFAITLKSYIECNRPQPAHRFLFLKIMAMLTELRSINAQHTQRLLRIQDIHPFATPLMQELFGITGSLVPRGSSSHSSLTERHKILHRLLQEGSPS |
| Amino-acid sequence of the protein after thrombin cleavage (PXRLBD)† | MKKGHHHHHHGSERTGTQPLGVQGLTEEQRMMIRELMDAQMKTFDTTFSHFKNFRLPGVLSSGCELPESLQAPSREEAAKWSQVRKDLCSLKVSLQLRGEDGSVWNYKPPADSGGKEIFSLLPHMADMSTYMFKGIISFAKVISYFRDLPIEDQISLLKGAAFELCQLRFNTVFNAETGTWECGRLSYCLEDTAGGFQQLLLEPMLKFHYMLKKLQLHEEEYVLMQAISLFSPDRPGVLQHRVVDQLQEQFAITLKSYIECNRPQPAHRFLFLKIMAMLTELRSINAQHTQRLLRIQDIHPFATPLMQELFGITGSLVPR |
†
HHHHHH, His6 tag; LVPRGS, thrombin cleavage site.