Figure 7.

Human PDAC studies (scRNA and spatial studies)

(A) The waterfall plot denotes the maximum percentage change in target lesion size from the baseline; PR, partial response; and SD, stable disease (Methods S1).

(B) Kaplan-Meier survival curves for enrolled patients.

(C) Kaplan-Meier survival curves for progression-free survival.

(D) Percentage of cells observed in pre- and post-treatment samples from patient A and D.

(E) T cell exhaustion genes expression in the baseline and treated human biopsy samples.

(F) Comparison between CD8⁺ T cells and NK cells from TME (biopsy) and baseline level.

(G) Spatial analysis of liver metastases before and after therapy. Immunofluorescent stains of six markers covering various structural or immune characteristics. One region of each pre-treated sample is displayed. Markers displayed include DAPI (nuclear), β-actin (structural), E-cadherin (normal, PDAC tissue), collagen-IV (fibrotic), pan-cytokeratin (PCK, PDAC), and CD3e (T cell). A composite image including E-cadherin (green), collagen-IV (yellow), PCK (cyan), and CD3e (red).

(H) Cell annotations for select regions from pretreated samples A (left) and D (right). The left column shows the composite image with the same color scheme from (G). The right column displays the cell classification for each region.

(I) Adjacency analysis for the cell types annotated in (H) for pretreated samples A and D. Each square displays the log-odds ratio for finding a cell of one type adjacent to another. Red indicates a higher likelihood of being found near each other than random, and blue indicates a lower probability.

(J) Displaying individual fluorescent markers for select regions from post-treated samples A and D. The composite image uses the same color mappings as (G).

(K) A stacked barplot displays the percent area coverage of normal, fibrotic, and PCK+ malignant tissue for pre-treatment and post-treatment samples.

(L) Cell annotations for select regions from post-treated samples A and D, using the color scheme defined in (H).

(M) Adjacency analysis for the cell types annotated in (F) for the post-treated sample A (left) and D (right). See also Figure S12 and Tables S1 and S2.