Fig. 2.

Mouse skin was evaluated though Histological analysis. (A, B) (A) Representative images of Hematoxylin and eosin (H&E) staining in CTL, PBS, MSCs and Pime. Scale bars, 500 μm. (B) Quantification of epidermal thickness. n = 4 for each slide, mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001. (C, D) (C) Representative confocal images of CD31-positive cells (green) and nuclei (blue) stained with endothelial markers in the CTL, PBS, MSCs and Pime groups. Scale bars, 200 μm. (D) Quantification of marker CD31-positive cells. n = 3 for each slide, mean ± SD; * p < 0.05, *** p < 0.001. (E, F) (E) Representative images of Toluidine blue staining in CTL, PBS, MSCs and Pime. Scale bars, 500 μm. (F) Quantification of mast cell numbers per field. mean ± SD; ** p < 0.01, *** p < 0.001. (G, H) (G) Representative confocal images of MCT-positive cells (green) and nuclei (blue) stained with mast cell activation markers in CTL, PBS, MSCs and Pime. Scale bars, 200 μm. (H) Quantification of marker MCT-positive cells. n = 3 for each slide, mean ± SD; * p < 0.05, *** p < 0.001