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. 2005 May 10;388(Pt 1):7–15. doi: 10.1042/BJ20042033

Figure 2. 2-D gel electrophoresis for protein extracts from DNA-damage-induced nuclei.

Figure 2

Protein extracts, prepared as described in Figure 1, were subjected to 2-D gel electrophoresis with a pI range from 3 to 10 and 12.5% gels. The scanned images of silver-stained wet gels are shown. The salt concentrations used for protein extraction are indicated above each group of gels. On each of the gels in (C), the protein spots showing a large difference in their intensity between control and DSB-induced samples are numbered within a box. The indicated protein spots of the third gel (Etoposide) in (C) were excised for trypsin digestion and MALDI–TOF MS analysis.