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. 2005 May 10;388(Pt 1):17–28. doi: 10.1042/BJ20041236

Figure 1. MEKK4 is tyrosine-phosphorylated in an IFNγ-dependent manner in HaCaT cells.

Figure 1

(A) HaCaT cells deprived of serum were treated with 200 units/ml IFNγ as indicated, and MEKK4 was immunoprecipitated with antibodies directed against amino acids 18–139 of MEKK4 and immunoblotted (IB) for phosphotyrosine (α P-Y; upper panel). The total amount of protein was determined by re-probing with antibodies directed against amino acids 1102–1255 of MEKK4 (α MEKK4; lower panel). (B) Co-immunoprecipitation of Pyk2 and annexin II with MEKK4. The immunoblot was probed with monoclonal antibodies against Pyk2 (α Pyk2; upper panel) and annexin II (α annexin II; lower panel).