Table 2. ITC measurements to delineate the first Cdc42.GTP-binding region of Scd2.

Experimental conditions and units are as in Table 1.

	Kd	ΔH	TΔS
Scd2-(1–86)	3.00±1.01	−2.35±0.23	5.03±0.03
Scd2-(1–86) Trp62→Arg	No binding
Scd2-(24–86)	No binding
Scd2-(1–123)	0.017±0.002	−5.72±0.01	4.65±0.07
Scd2-(1–123) Trp62→Arg	0.074±0.002	−4.88±0.13	4.63±0.15