Table 3. ITC measurements to delineate the second Cdc42.GTP-binding region of Scd2.

Experimental conditions and units are as in Table 1.

	Kd	ΔH	TΔS
Scd2-(110–266)	2.93±0.40	1.91±0.01	9.28±0.09
Scd2-(188–266)	No binding
Scd2-(110–220)	1.71±0.15	2.55±0.38	10.14±0.42
Scd2-(110–220) Trp160→Arg	2.52±0.85	3.07±0.37	10.55±0.16