Table 3. ITC measurements to delineate the second Cdc42.GTP-binding region of Scd2.
Kd | ΔH | TΔS | |
---|---|---|---|
Scd2-(110–266) | 2.93±0.40 | 1.91±0.01 | 9.28±0.09 |
Scd2-(188–266) | No binding | ||
Scd2-(110–220) | 1.71±0.15 | 2.55±0.38 | 10.14±0.42 |
Scd2-(110–220) Trp160→Arg | 2.52±0.85 | 3.07±0.37 | 10.55±0.16 |