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. 2005 May 10;388(Pt 1):195–204. doi: 10.1042/BJ20041797

Figure 1. Generation of mutant stoned flies with a reduction in the levels of DAP-160.

Figure 1

(A) Quantitative estimates of DAP-160 levels were determined for the various strains. 5, 10 and 20 heads from a white-eyed Canton-S ‘wild-type’ strain, homozygous EP(2)2543 flies and EP(2)2543/Df(2)TW65 heterozygotes were homogenized directly into SDS/PAGE sample buffer, centrifuged to remove particulates, and loaded on to SDS/PAGE (7% gel). The resulting Western blot was then probed with anti-DAP-160 antibody showing that EP(2)2543 reduced DAP-160 levels by 50% and EP(2)2543/Df(2)TW65 to 25% of the wild-type levels. (B) A diagram of the crosses performed to produce flies that carry a stoned mutation and have different levels of DAP-160. Only the males exhibit the stoned phenotypes and one group of these males will also have 25% of the normal DAP-160 levels. (C) The relative viability of the progeny from the cross outlined in (B) using female parents that are w stnts; EP(2)2543 homozygotes were determined. The asterisked group of flies are the w stnts/Y; EP(2)2543/Df(2)TW65 males. (D) The relative viability of the progeny from the cross outlined in (B) using female parents that are w stnC; EP(2)2543 homozygotes. The asterisked group of flies are the w stnC/Y; EP(2)2543/Df(2)TW65 males.