Figure 1. Nucleotide sequences (cDNA) of SGPP-4 isoforms.

(A) Nucleotide sequence (cDNA) and deduced amino acid sequence (in bold) of SGPP-4a. The signal peptide sequence is in italic, and its computed cleavage site [32] is indicated by an arrowhead (▼). The predicted dibasic cleavage site between two PLD-related domains is underlined. 5′- and 3′-RACE primers are indicated as grey and black arrows respectively. Ala⁶⁶, which differs from the SGPP-4t amino acid sequence, is boxed. A putative polyadenylation signal sequence is indicated in bold and underlined. The nucleotide regions, from which the real-time primers were derived, are boxed. Finally, the nucleotide sequences, corresponding to the primers for the control PCR, are shaded (grey). (B) Nucleotide sequence (cDNA) of SGPP-4t. Nucleotides which differ from the SGPP-4a cDNA sequence are shaded. The codon, encoding Thr⁶⁶, is boxed.