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. 2005 May 10;388(Pt 1):291–297. doi: 10.1042/BJ20041568

Figure 4. Competitive mobility shift and UV cross-linking of 35-base fragment 4 with different RNA.

Figure 4

(A) Competition of different RNA (500 ng/20 μl) with radiolabelled fragment 4 (5 ng/20 μl) for mobility shift by protein from LVM nuclear extracts (2 μg/20 μl). R, radiolabelled RNA alone; RP, radiolabelled RNA+protein, but no unlabelled RNA. Protein was included in all the lanes with unlabelled RNA. The amount of unlabelled RNA was 500 ng/20 μl. The identities of the RNA fragment, given as numbers B1-3, B1-4, B1-5 and B1-4S, are as in Figure 3(B). Unlabelled 2B1 RNA (5 or 100 ng/20 μl) was used as a positive control. Arrows indicate the position of the radiolabelled RNA in the absence or the presence of the proteins from the nuclear extracts. (B). UV cross-linking of radiolabelled fragment 4 (2 ng/20 μl) with proteins (8 μg/20 μl) from LVM nuclear extracts. R, radiolabelled RNA alone; RP, radiolabelled RNA+protein alone. Competition with specified unlabelled RNA fragments (2.5 μg/20 μl) is shown in the right-hand three lanes.