Table 1. Binding properties of wild-type and mutant human FP-Rs.
Kd values were determined by non-linear regression analysis of saturation binding assays with membranes of cells expressing the respective receptor construct. The maximum specific binding capacity Bmax was calculated from specific binding at a single concentration (5 nM) taking into account the Kd value. Values are means±S.E.M. for at least three independent experiments. WT, wild-type.
| Receptor | Kd (nM) | Bmax (fmol/mg) |
|---|---|---|
| hFP WT | 13.4±6.0 | 2560±366 |
| hFP 318Stop | 13.4±2.9 | 1130±89 |
| hFP E132D | 9.8±1.6 | 5450±1010 |
| hFP C134Y | 13.7±3.0 | 1942±521 |
| hFP E132D,C134Y | 9.5±1.7 | 4211±709 |
| hFP E132T | 14.2±4.7 | 2563±283 |