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. 2005 May 10;388(Pt 1):333–342. doi: 10.1042/BJ20050057

Figure 4. Anti-PDE antibodies recognize specific PDEs.

Figure 4

V5-tagged constructs for PDE1, PDE6, PDE9 and PDE11 were transfected into S2 cells, and cell extracts prepared from transfected cells (T) and untransfected controls (C). Western blotting was performed with cell extracts using polyclonal anti-PDE antibodies. Left panel: (A) PDE1, (B) PDE6, (C) PDE9 and (D) PDE11. Right panel: anti-V5 antibody [18] (A–D) PDE1–PDE11, same as left panel. Bands identified by both anti-PDE and anti-V5 antibodies in samples from transfected but not control cells were as follows: PDE1: 75 kDa; PDE6: 150 kDa; PDE9: 47 kDa; PDE11: 100 kDa, indicated by use of molecular mass standards (kDa).