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. 2005 May 10;388(Pt 1):355–362. doi: 10.1042/BJ20041447

Figure 4. The proposed Kv1 trafficking code is not transferable to the Kv2.1 potassium channel.

Figure 4

Transiently transfected CHO cells were assayed for cell-surface protein levels as described in the legend to Figure 2. (A) Cell-surface protein profile of Kv2.1 constructs with different amino acid pairs in the deep pore by biotinylation/immunoblotting. (B) Group data for cell-surface expression. Normalized cell-surface protein levels by biotinylation/immunoblotting. The Kv2.1Y380K construct value was taken as 100.0±S.E.M. and the other construct values were normalized to it (n=3). (C) Total protein levels of cells by immunoblotting (immunoblots not shown). The Kv2.1Y380K construct value was taken as 100.0±S.E.M. and the other construct values were normalized to it (n=3). (D) Relative surface expression levels obtained by dividing surface values in (B) by total values in (C).