Figure 1.

Drs2 is required for CUPS biogenesis. (A) Cells genomically expressing Drs2-3xGFP and Grh1-2xmCherry were visualized by confocal spinning-disk microscopy in growth conditions and starvation by incubation in 2% potassium acetate. Short movies were acquired at 10-s intervals to assess the frequency and duration of colocalization. Scale bar = 2 μm. (B) WT and drs2Δ cells expressing Grh1-2xGFP were visualized by epifluorescence microscopy in growth conditions and after incubation in 2% potassium acetate for the indicated times. Cells were classified with normal CUPS (one to three larger foci per cell); intermediate CUPS (“int.”), where a large focus is observed in addition to smaller structures; and vesiculated CUPS (“ves.”), where only small foci of Grh1 are observed. Scale bar = 2 μm.