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. 2000 Feb;53(1):37–42. doi: 10.1136/mp.53.1.37

Figure 2.

Figure 2

Validation of cytoblock techniques. (A) In situ hybridisation with 35S labelled antisense probes reveals expression of Epstein-Barr virus (EBV) encoded small RNAs (EBERs) in most B95.8 cells. (B) A small proportion of B95.8 cells is also labelled with the sense control probes, indicating hybridisation to replicating viral DNA (see text). (C) In situ hybridisation with a probe specific for the BamHI W fragment of the EBV genome results in the labelling of a proportion of B95.8 cells, whereas the EBV negative Ramos cells (D) are unlabelled. (E) In situ hybridisation with a 35S labelled U6 specific RNA probe results in the accumulation of silver grains over scattered small inflammatory cells, whereas nuclei of epithelial cells are negative. (F) In situ hybridisation with a digoxigenin labelled Cot1 specific DNA probe results in red nuclear staining of most epithelial and inflammatory cells. (G) EBER specific in situ hybridisation with 35S labelled RNA probes shows no evidence of latent EBV infection in desquamated oropharyngeal epithelial or inflammatory cells from a patient with acute infectious mononucleosis. (H) Using a 35S labelled probe specific for the BamHI W fragment of the EBV genome, viral DNA is not detected in desquamated oropharyngeal cells.