Figure 3.

Molecular biological detection of of c-kit Asp 816 to Val point mutation. Semi-nested PCR products from control DNA (lane 1) and three different microdissected mast cell infiltrates (lanes 2–4). In lane 1, the undigested full length PCR product is shown. Lanes 2–4 contain HinfI digested PCR products (table 2 ▶). In lane 2 only the mutated allele is present, most probably indicating loss of heterozygosity (LOH). Signals from both the wild-type and mutated allele are present in lane 3 (heterozygous genotype). Lane 4 contains the wild-type sequence (homozygous genotype). In lane 5 only water was amplified as a negative control. M, HaeIII digested φX DNA.